益气活血利水法治疗心力衰竭水潴留机制的实验研究
益气活血利水法治疗心力衰竭水潴留机制的实验研究
An experimentalstudy on water retention mechanism of
Heart Failure by theYiqi Huoxue Lishui therapy
刘淑荣 张晓华※ 邸琳 刘新宇
（吉林省中医药科学院，吉林 长春，130021）
中图分类号：R289.5 文献标识码：A 文章编号：1674-7860(2015)18-0062-02 证型：DGBA
【摘 要】目的：探讨益气活血利水法治疗心衰水潴留的机制。方法：选择健康Wistar大鼠，应用腹腔注射阿霉素，造成心衰大鼠模型，然后分模型组、西药对照组，中药对照组、中药高剂量、中剂量、低剂量6组，给药8周后观察各组大鼠服药后血管加压素（AVP）浓度；血管加压素V2受体（V2R）、水通道蛋白2（AQP-2）蛋白表达及mRNA表达情况。结果：益气活血利水法可降低心衰大鼠血浆中AVP浓度，减弱肾组织V2R、AQP-2蛋白表达，降低V2R、AQP2、mRNA相对表达量。结论：益气活血利水法通过作用于精氨酸血管加压素-V2受体-水通道蛋白2系统的各个环节而起到改善水液潴留的作用。
【关键词】心力衰竭；益气活血利水法；血管加压素；水通道蛋白2
【Abstract】Objective:To investigate the Yiqi Huoxue Lishui therapy for the water retention mechanismof heart failure. Methods: Healthy Wistar rats, with intraperitoneal injectionof doxorubicin, resulting in a rat model of heart failure, and then were divideinto the model group, western medicine group,TCM control group, high dose,medium-dose,low dose six groups administered 8 weeks after the rats aftertaking vasopressin (AVP) concentration; vasopressin V2 receptor (V2R),aquaporin-2 (AQP-2) protein and mRNA expression. Results Yiqihuoxue therapy can reduceheart failure in rat plasma AVP concentration, decreased kidney tissue V2R, AQP-2protein, lower V2R, AQP2,mRNA relative expression.Conclusion: Yiqihuoxue therapyby acting on arginine vasopressin receptor -V2 - all aspects of aquaporin 2system and play a role in improving water retention.
【Keywords】Heartfailure;Yiqihuoxue therapy;Vasopressin;Aquaporin 2
doi:10.3969/j.issn.1674-7860.2015.18.031
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