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加味六君子汤对腹膜纤维化大鼠TGF-β/Smad信号通路 的影响

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孟立锋  向彩春  杨端云  陶志虎  覃志周  农邵阳

(广西中药大学第一附属医院,广西  南宁,530023

中图分类号:R289.6       文献标识码:A    文章编号:1674-7860(2016)12-0018-03   证型:IDGB

 】目的:探讨加味六君子汤对腹膜纤维化大鼠TGF-β/Smad信号通路的影响。方法:用5/6肾切除联合腹腔注射4.25%腹透液+脂多糖(LPS)建立腹膜纤维化大鼠模型,实验分空白组(A组)、模型组(B组)、西药组(C组)、中药高剂量组(D组)、中药低剂量组(E组),Western印迹法检测TGF-β1p-Smad2/3Smad7α-SMAE-cadherin蛋白的表达:RT-PCR法测定TGF-β1α-SMAE-cadherinmRNA水平。结果:与A组相比,B组、C组、D组、ETGF-β1p-Smad2/3α-SMA蛋白和α-SMAmRNA表达水平升高,有显著性差异(P0.05);与B组相比,C组、D组、ETGF-β1p-Smad2/3α-SMA蛋白和α-SMAmRNA表达水平降低,Smad7E-cadherin蛋白和E-cadherinmRNA表达水平增高,有显著性差异(P0.05)。结论:加味六君子汤可能通过TGF-β/Smad信号通路,抑制腹膜间皮细胞EMT进程,进而起到一定拮抗腹膜纤维化作用。

关键词】腹膜纤维化;TGF-β/Smad信号通路;腹膜间皮细胞间充质转化;加味六君子汤

AbstractObjective: Toinvestigate the effect of Modified Liujunzi decoction on TGF-β/Smad singnal pathway of peritonealfibrosis rat model. Methods: The peritoneal fibrosis rat model was establishedby 4.25% dialysate and lipopoly saccharide (LPS) based on five sixthsnephrectomyThe rats were randomly divided into controlgroup (A)peritoneal fibrosis model group (B), westernmedicine group (C), high dose Modified Liujunzi decoction treating group (D),low dose Modified Liujunzi decoction treating group (E).The expression of TGF-β1, p-Smad2/3, Smad7, α-SMA and E-cadherin protein inperitoneal membrane was detected with Western blotting, the level of TGF-β1,α-SMA and E-cadherin mRNA was detected by using real-time polymerase chain reaction (RT-PCR)ResultsAfter treatment, compared with group A, the expressionof TGF-β1, p-Smad2/3,α-SMA protein andα-SMAmRNA in groupB, C, D and E was up-regulated,and the difference was statistically significant (P<0.05).Compared with group B, the expression of TGF-β1, p-Smad2/3, α-SMA protein andα-SMAmRNA in group C,D and E decreased while the expression of Smad7,E-cadherin protein and E-cadherin mRNA increased, and the difference was statistically significant (P<0.05). Conclusion: Modified Liujunzi decoction could preventor reduce the incidence and progression of epithelial-to-mesenchymal transitionin peritoneal fibrosis through TGF-β/Smadsingnal pathway, which in turn exerted a certain antagonism of peritoneal fibrosis.

KeywordsPeritonealfibrosis; TGF-β/Smad singnal pathway;Epithelial-to-mesenchymal transition; Modified Liujunzi decoction

doi:10.3969/j.issn.1674-7860.2016.12.009

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